FastQCFastQC Report
Mon 6 Jun 2016
ERR1211608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996582
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207060.41440328608636867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180900.3620474956680387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129170.2585167220311805No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92010.18414588212502064No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55490.11105591782542545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG67250.053.074512
GTACCTG69850.052.435751
ACCTGGG80300.043.595583
GGTATCA102450.042.0079651
GTATCAA161400.035.2514231
TATAACG6000.033.7018472
CCTGGGG71200.031.5463874
TAACGCA7600.027.8227864
TCAACGC200450.027.7788354
CAACGCA203200.027.4031645
ATCAACG203650.027.3192653
TATCAAC210550.026.4890212
AACGCAG212700.026.378596
GTACATG180100.025.8069131
ATAACGC8650.024.9886883
TACATGG183600.024.7167382
ACATGGG180500.024.1846733
ACGCAGA240700.023.2124257
CATGGGG109450.023.1835384
CGCAGAG247700.022.3857048
Produced by FastQC (version 0.11.5)