FastQCFastQC Report
Mon 6 Jun 2016
ERR1211607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5772840
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174690.30260668925520195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.25238877225074663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100730.17448950603169325No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59850.10367514083189558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72550.047.6683162
GGTATCA88100.046.8492241
GTACCTG76150.046.210341
ACCTGGG87300.039.3482743
GTATCAA147100.036.4601171
TCAACGC177350.029.6484934
CAACGCA179750.029.2234525
ATCAACG179950.029.1430443
GTACATG175650.028.922631
AACGCAG186300.028.4684896
TATAACG6900.027.958552
TATCAAC188200.027.90132
TACATGG180000.027.6039522
ACATGGG181500.026.4345343
CCTGGGG85250.026.0717664
ACGCAGA209650.024.8274427
TAACGCA6500.024.5792884
CATGGGG117300.024.4763664
CGCAGAG217050.023.8958638
ATAACGC7900.022.6036743
Produced by FastQC (version 0.11.5)