FastQCFastQC Report
Mon 6 Jun 2016
ERR1211607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5772840
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200040.34651921757748355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169680.2939281185690232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120070.20799121402983625No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.11001517450682852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73650.049.173012
GTACCTG76100.048.2675131
GGTATCA97400.044.0539171
ACCTGGG85750.041.537943
GTATCAA162450.037.7624131
TAACGCA6850.030.8696614
CCTGGGG81700.029.505654
TCAACGC204250.029.3331034
TATAACG7250.029.193312
ATCAACG205950.029.113793
CAACGCA206550.029.0287155
AACGCAG215600.027.984576
TATCAAC215450.027.9211242
GTACATG185750.025.3340051
ACGCAGA245050.024.4867447
ATAACGC7900.024.3874653
TACATGG190800.023.8619422
CGCAGAG252200.023.643488
ACATGGG188350.023.2020953
CATGGGG118900.022.2898484
Produced by FastQC (version 0.11.5)