FastQCFastQC Report
Mon 6 Jun 2016
ERR1211606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6854518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195030.28452766481902886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169190.24682990109589031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114010.16632825240228416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.045.8238641
GTATCAA176600.035.8461571
TACCTGG60250.030.8464782
GTACCTG64350.029.9816531
TCAACGC209450.029.185444
CAACGCA211800.028.881065
ATCAACG212600.028.7090233
GTACATG218600.028.0526581
AACGCAG219900.028.0066536
TATCAAC228950.026.8185562
TACATGG223300.026.5910912
ACATGGG224200.025.5051253
ACGCAGA251000.024.2375857
ACCTGGG76550.024.1223623
CGCAGAG256650.023.5946988
CATGGGG122650.022.985454
GCAGAGT287550.020.307699
GAGTACT180100.019.10969512-13
AGAGTAC259950.019.0228810-11
ATGGGGG73450.018.869415
Produced by FastQC (version 0.11.5)