FastQCFastQC Report
Mon 6 Jun 2016
ERR1211606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6854518
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227580.3320145924191898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201000.2932372487751874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136110.19856976084970523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122100.046.337731
GTATCAA201900.034.561661
TACCTGG58350.033.2024352
GTACCTG60850.032.3105741
TCAACGC246300.027.5857454
ATCAACG248400.027.352933
CAACGCA250000.027.1968655
AACGCAG259050.026.3381986
GTACATG235200.026.0199571
TATCAAC262900.026.006832
ACCTGGG73900.025.4332983
TAACGCA6750.025.0599124
TACATGG238350.024.8384322
ACATGGG237100.024.118273
ACGCAGA292600.023.1574527
CGCAGAG299650.022.5812538
CATGGGG128650.022.2062194
TATAACG7200.021.552372
GCAGAGT334000.019.9212519
GAGTACT209900.019.68826312-13
Produced by FastQC (version 0.11.5)