FastQCFastQC Report
Mon 6 Jun 2016
ERR1211605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6540571
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171210.2617661363205139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147490.22550018950944803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98190.1501245074780168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99250.043.0523831
GTATCAA163000.035.9363171
GTACCTG57400.034.0985371
TACCTGG59550.032.071942
TCAACGC191350.029.4433044
CAACGCA193850.028.9650765
ATCAACG195400.028.8330383
AACGCAG201050.028.1102286
TATCAAC205150.027.5851332
GTACATG204950.027.5221771
TACATGG209950.026.013412
ACATGGG210850.025.0934643
ACCTGGG75950.024.809183
ACGCAGA229000.024.433137
CATGGGG126600.023.6429424
CGCAGAG236000.023.550078
GCAGAGT264200.020.6804379
GAGTACT157050.019.50662812-13
ATGGGGG79500.019.2673955
TAACGCA6600.018.5108014
Produced by FastQC (version 0.11.5)