FastQCFastQC Report
Mon 6 Jun 2016
ERR1211605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6540571
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198780.3039184193551297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173780.26569545686454593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117980.18038180458556294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111350.044.388631
GTACCTG58600.036.3782271
TACCTGG58950.035.501422
GTATCAA182200.035.4369051
TCAACGC222200.028.0852414
ATCAACG225200.027.7323933
CAACGCA224950.027.6996965
ACCTGGG76650.026.7916853
AACGCAG235050.026.589626
TATCAAC238750.026.3758662
GTACATG214400.025.6052421
TACATGG220050.024.2787592
CATGGGG131100.023.3705544
ACGCAGA266200.023.3367777
ACATGGG220950.023.1826573
CGCAGAG272800.022.6515988
ATGGGGG76950.020.2132825
GCAGAGT306000.019.8100579
GAGTACT190550.019.11232612-13
AGAGTAC283550.018.92578510-11
Produced by FastQC (version 0.11.5)