FastQCFastQC Report
Mon 6 Jun 2016
ERR1211604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8057146
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193300.23991125393532647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171160.21243254124971794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105610.13107619000574147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113400.046.7153131
GTATCAA195250.035.5397381
TCAACGC223300.029.9642754
CAACGCA225750.029.6779465
ATCAACG226700.029.5568793
AACGCAG235050.028.5597726
GTACATG240750.027.9608421
TATCAAC247350.027.4125692
TACATGG248800.026.723262
ACATGGG243700.025.9332753
GTACCTG65700.025.273761
ACGCAGA267600.024.840247
TACCTGG66400.024.590032
CGCAGAG274550.024.1090688
CATGGGG134750.022.07284
GCAGAGT310650.020.5664069
ACCTGGG79800.019.7845883
GAGTACT191050.019.31658712-13
AGAGTAC281300.019.12265410-11
CAGAGTA301200.018.4909410-11
Produced by FastQC (version 0.11.5)