FastQCFastQC Report
Mon 6 Jun 2016
ERR1211604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8057146
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212160.2633190462230671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192100.23842189281415527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127990.15885277491558425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125000.045.680481
GTATCAA204900.035.024281
TCAACGC243700.028.4983524
CAACGCA247050.028.149435
GTACCTG63800.028.084031
ATCAACG248800.027.9523013
AACGCAG258000.027.0098556
GTACATG249000.026.2837091
TATCAAC268250.026.1578462
TACCTGG67950.025.9546222
TACATGG258150.025.1226272
ACATGGG252050.024.2361073
ACGCAGA295500.023.470757
CGCAGAG305150.022.6207268
CATGGGG141500.021.3528274
ACCTGGG83450.020.2150253
GCAGAGT342800.019.7385129
GAGTACT212950.019.56118612-13
AGAGTAC312100.018.93245510-11
TATAGCG3358.4764906E-1018.234395
Produced by FastQC (version 0.11.5)