FastQCFastQC Report
Mon 6 Jun 2016
ERR1211603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8115858
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230420.28391329665945364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200510.24705952223412486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132110.16278007821230978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126250.047.304721
GTATCAA210900.037.7123151
TCAACGC254700.030.4762464
CAACGCA255750.030.2922735
ATCAACG257200.030.0881143
AACGCAG267800.029.0656416
TATCAAC274050.028.3442842
GTACATG264850.028.1957171
TACATGG268300.027.3384342
TACCTGG67550.026.5367552
ACATGGG270900.026.3637353
GTACCTG72000.026.076711
ACGCAGA302450.025.4100597
CATGGGG163950.024.6185324
CGCAGAG310750.024.5210938
GCAGAGT350550.021.0133919
TATAACG7950.020.7132912
ACCTGGG85600.020.3644433
ATGGGGG105300.020.2114145
GAGTACT213600.020.12686312-13
Produced by FastQC (version 0.11.5)