FastQCFastQC Report
Mon 6 Jun 2016
ERR1211603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8115858
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260220.32063153396720345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233760.28802869641139606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157650.1942493326029115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143000.045.104881
GTATCAA233650.036.0201871
TACCTGG63050.029.9822442
GTACCTG68450.028.4478911
TCAACGC289700.028.415514
ATCAACG292700.028.1563743
CAACGCA293000.028.1278925
AACGCAG304700.027.1260996
TATCAAC310500.026.7304522
GTACATG277000.026.3660551
TACATGG285600.025.0928482
ACATGGG285950.024.4337833
CATGGGG164950.024.0698974
ACGCAGA344900.023.8277237
CGCAGAG351600.023.2801198
ACCTGGG81600.022.571783
ATGGGGG96350.021.8474865
TAACGCA7650.021.4969314
GCAGAGT391500.020.4154269
TATAACG8250.019.9497432
Produced by FastQC (version 0.11.5)