FastQCFastQC Report
Mon 6 Jun 2016
ERR1211602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537848
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309070.21259680249786625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255920.1760370585797843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170180.11705996650948614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188100.045.4088671
GTATCAA337050.037.067951
ATCAACG373750.031.7065563
TCAACGC374200.031.693544
CAACGCA378450.031.2719925
AACGCAG392750.030.2253366
GTACATG462700.028.673531
TATCAAC427450.028.0776352
TACATGG471300.027.252142
ACATGGG458200.027.154893
ACGCAGA451200.025.9353837
CGCAGAG456000.025.5088148
GTCCTAC80400.022.584771
CATGGGG201100.021.7999134
GCAGAGT524400.021.3482049
CATGGGA250950.020.7087144
GTACCTG95200.020.3617861
AGAGTAC481050.018.95693810-11
GAGTACT312800.018.84313812-13
GTATAGG61000.018.7882981
Produced by FastQC (version 0.11.5)