FastQCFastQC Report
Mon 6 Jun 2016
ERR1211602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537848
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353800.24336476760521913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303080.20847652279759699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210390.1447188056994405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219250.042.1967661
GTATCAA373950.035.8242761
ATCAACG429700.030.0725213
TCAACGC432000.029.9449394
CAACGCA438200.029.5534275
AACGCAG455950.028.5069586
GTACATG482800.027.8060911
TATCAAC483650.026.9542732
ACATGGG475950.026.4196623
TACATGG496550.026.3582042
ACGCAGA520350.024.8250777
CGCAGAG521200.024.577318
CATGGGG204150.023.75374
GCAGAGT596800.021.022969
AGAGTAC549250.019.36526310-11
GAGTACT361550.019.04690612-13
GTACCTG95450.018.8715321
GTCCTAC78500.018.5613081
CATGGGA264050.018.4719244
ATGGGGG114350.018.1218875
Produced by FastQC (version 0.11.5)