FastQCFastQC Report
Mon 6 Jun 2016
ERR1211601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9186504
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185370.20178514046257423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160800.1750393838613688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103510.11267616059384507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115850.045.347971
GTATCAA201950.037.19981
TCAACGC236700.030.5505854
CAACGCA239600.030.119365
ATCAACG240500.030.0287953
GTACATG282000.029.2990591
AACGCAG248100.029.2919396
TATCAAC259400.028.1292442
TACATGG289400.027.5866872
ACATGGG289900.026.2894673
ACGCAGA285650.025.0634047
GTACCTG78550.025.035631
CGCAGAG296900.023.9250148
TACCTGG80800.023.2897512
CATGGGG175950.022.5655844
GCAGAGT339450.020.137039
AGAGTAC299400.018.8469910-11
GAGTACA205250.018.6569881
GAGTACT195250.018.64996512-13
ATGGGGG103750.018.5668035
Produced by FastQC (version 0.11.5)