FastQCFastQC Report
Mon 6 Jun 2016
ERR1211601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9186504
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220370.23988450884035972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195290.21258359001422086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129440.14090234979487298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136300.043.6142431
GTATCAA225500.035.978531
TCAACGC267700.029.402284
CAACGCA270500.029.0807175
ATCAACG272750.028.806523
AACGCAG285350.027.6834986
GTACATG301400.027.6064511
TATCAAC289700.027.254362
TACATGG312550.025.8937632
ACATGGG311550.025.1134173
GTACCTG76650.024.5397321
ACGCAGA329800.023.766737
CGCAGAG339400.023.0114088
TACCTGG80200.022.9264182
CATGGGG182100.022.5794164
GCAGAGT387000.019.7073969
ATGGGGG103000.019.1614785
GAGTACT224300.018.81346112-13
ACCTGGG97400.018.5746693
AGAGTAC349000.018.47359810-11
Produced by FastQC (version 0.11.5)