FastQCFastQC Report
Mon 6 Jun 2016
ERR1211600_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211600_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020767
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237640.2634365791733674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202760.22477024403800694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132240.14659507334575875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131250.044.9961551
GTATCAA220650.038.8010141
TCAACGC269900.030.8519974
CAACGCA271300.030.6727475
ATCAACG274150.030.374223
AACGCAG282700.029.6950426
TATCAAC286600.029.156882
GTACATG307700.028.8511091
TACATGG317200.027.6049822
GTACCTG79000.026.2721711
ACATGGG322200.026.223693
TACCTGG76750.026.1772352
ACGCAGA327750.025.427347
CATGGGG217600.024.5972924
CGCAGAG338850.024.331798
GCAGAGT381650.020.9752129
ATGGGGG128300.020.4357915
ACCTGGG99300.019.8287093
AGAGTAC337400.019.37756310-11
GAGTACT220000.019.13647712-13
Produced by FastQC (version 0.11.5)