FastQCFastQC Report
Mon 6 Jun 2016
ERR1211600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020767
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266310.29521879902230047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229460.2543686141100862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157400.1744862715110589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146400.046.0631331
GTATCAA244450.037.2328071
TCAACGC300900.029.5606884
ATCAACG305250.029.186423
CAACGCA305250.029.170385
TACCTGG78250.028.9714552
GTACCTG83050.028.5618841
TATCAAC318250.028.2275092
AACGCAG317600.028.1852786
GTACATG315600.026.5527151
TACATGG324800.025.283422
ACATGGG329200.024.265393
ACGCAGA368750.024.1097167
CGCAGAG382100.023.132218
CATGGGG213300.022.9101854
ACCTGGG102850.021.5186393
TATACCG8950.021.00035
TATAACG8350.020.2779312
GCAGAGT426000.020.218829
ATGGGGG120200.020.1322025
Produced by FastQC (version 0.11.5)