FastQCFastQC Report
Mon 6 Jun 2016
ERR1211599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401373
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158490.2934253938767051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136000.25178783246407904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91950.17023449408141228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85250.043.7186741
TACCTGG57650.040.0751462
GTACCTG59350.039.983571
GTATCAA141850.036.4847681
TATAACG5150.034.7191662
ACCTGGG68950.032.6381953
TCAACGC169850.029.845914
ATCAACG171350.029.584363
CAACGCA171600.029.539625
TATCAAC180500.028.3103712
AACGCAG181200.028.1003366
TAACGCA6700.028.0487864
GTACATG164650.026.905321
TACATGG169750.025.557252
ACATGGG166700.024.7729743
ACGCAGA205950.024.5413787
ATAACGC7800.024.0929663
CGCAGAG210100.023.856298
CATGGGG107450.023.4362034
CCTGGGG64550.021.8350354
Produced by FastQC (version 0.11.5)