FastQCFastQC Report
Mon 6 Jun 2016
ERR1211599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401373
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184370.3413391372897224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158060.2926292999946495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112880.2089839009451856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.10412167424838092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87600.045.715961
GTACCTG60950.042.7972341
TACCTGG62300.041.219572
GTATCAA150550.037.2846831
ACCTGGG71850.034.7931333
TCAACGC186650.029.505594
ATCAACG187150.029.4270343
TAACGCA5450.029.314814
CAACGCA189100.029.1973195
TATCAAC196800.028.2004362
AACGCAG198500.028.1237166
TATAACG5800.027.570832
GTACATG175500.026.312971
TACATGG179850.025.4972322
ACATGGG174000.025.2505663
CCTGGGG65500.025.0373764
ACGCAGA224150.024.7167787
CGCAGAG230150.024.0111588
CATGGGG114600.023.4539574
ATAACGC7400.022.2251663
Produced by FastQC (version 0.11.5)