FastQCFastQC Report
Mon 6 Jun 2016
ERR1211598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926043
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200950.2535313018110046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175110.2209299142081364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109690.1383918810432898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114400.045.6249661
GTATCAA190700.037.062261
TCAACGC223250.030.4156324
ATCAACG224600.030.2124633
CAACGCA227050.029.9238825
AACGCAG236800.028.9051346
TATCAAC241800.028.4242362
GTACATG243950.027.194071
TACATGG249550.025.7506372
ACGCAGA272650.024.7089637
ACATGGG251900.024.699523
CGCAGAG278500.023.8534688
GTACCTG63000.022.9013881
TACCTGG61200.022.5222112
CATGGGG150500.022.1688084
GCAGAGT311350.020.3709959
GAGTACT192650.019.18345812-13
GAGTACA181400.018.4284951
CAGAGTA301850.018.21257410-11
AGAGTAC281850.018.1712610-11
Produced by FastQC (version 0.11.5)