FastQCFastQC Report
Mon 6 Jun 2016
ERR1211598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926043
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221230.2791178397593856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204040.25742984235639393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127490.16084949324650397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125450.045.238191
GTATCAA206100.037.6902161
TCAACGC250050.030.5563344
CAACGCA253750.030.1104035
ATCAACG253750.030.0370883
AACGCAG264250.028.9497096
TATCAAC270100.028.4812812
GTACATG273200.025.1378561
ACGCAGA305050.024.923537
TACATGG273850.024.433912
CGCAGAG315150.024.0353138
ACATGGG275250.023.4740723
GTACCTG65100.022.5179481
CATGGGG163300.022.4160394
TACCTGG65600.021.6473082
GCAGAGT350650.021.4004149
GAGTACT206750.019.97725712-13
AGAGTAC319550.019.47620210-11
CAGAGTA338400.018.51628110-11
AGTACTT221750.018.44580712-13
Produced by FastQC (version 0.11.5)