FastQCFastQC Report
Mon 6 Jun 2016
ERR1211597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778309
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227410.2923643172314188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186600.23989790068766875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125280.16106328509191392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118550.046.7920881
GTATCAA202150.037.684731
TACCTGG69400.031.187962
GTACCTG72550.031.078291
TCAACGC244500.030.4984264
CAACGCA246900.030.1029385
ATCAACG248650.029.932523
GTACATG279450.029.4549271
AACGCAG258600.028.8831546
TATCAAC261900.028.6379172
TACATGG288700.027.9352552
ACATGGG295300.026.4450363
CATGGGG183350.024.9607374
ACGCAGA299800.024.6320047
CGCAGAG306450.023.8837998
ACCTGGG88300.023.6796843
TATAACG8100.022.0743122
ATGGGGG107850.021.86795
TAACGCA8450.021.6863924
GCAGAGT341450.020.8715179
Produced by FastQC (version 0.11.5)