FastQCFastQC Report
Mon 6 Jun 2016
ERR1211597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778309
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251850.3237850283397073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214400.27563831676010814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150670.19370534135375697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139600.043.2328341
GTATCAA221550.035.4349371
TACCTGG71750.032.118312
GTACCTG77550.030.886671
GTACATG293300.027.8918041
TCAACGC277200.027.6652094
CAACGCA280600.027.2963245
ATCAACG280800.027.2944953
TACATGG305250.026.43872
TATCAAC291850.026.4117852
AACGCAG293850.026.1786216
ACATGGG305850.025.5045593
CATGGGG190850.024.6707614
ACCTGGG91500.024.599863
ACGCAGA337150.022.6491137
CGCAGAG346100.021.9547988
ATGGGGG105500.020.4439335
GCAGAGT387650.019.5530979
GAGTACT216500.019.30643512-13
AGAGTAC346550.019.06515910-11
Produced by FastQC (version 0.11.5)