FastQCFastQC Report
Mon 6 Jun 2016
ERR1211596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898170
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172520.3522131734913243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149880.30599182960166754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98310.20070761120990085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.11681913857624379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59750.052.828362
GTACCTG62750.051.1027761
ACCTGGG67950.045.502053
GGTATCA99100.041.5422441
GTATCAA164950.033.1345021
TATAACG4500.030.3157312
TAACGCA5050.029.7744834
CCTGGGG53600.028.4031074
GTACATG199400.027.93021
TCAACGC192450.027.3210744
ATCAACG191700.027.2814563
CAACGCA194250.027.0173235
ACATGGG197450.026.6773683
TACATGG206550.026.4872632
AACGCAG200550.026.282266
TATCAAC207100.025.4401952
CATGGGG84100.025.4214924
ACGCAGA226800.023.074867
CGCAGAG229100.022.7621128
ATGGGGG54900.021.6520165
Produced by FastQC (version 0.11.5)