FastQCFastQC Report
Mon 6 Jun 2016
ERR1211596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898170
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198290.40482465900530196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176030.35937911505725606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121980.24903178125708172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61530.12561834317714574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58150.052.964662
GTACCTG60450.051.3976361
ACCTGGG68050.044.4015433
GGTATCA108650.042.3086281
TAACGCA6200.036.378324
TATAACG6350.035.543662
GTATCAA178750.033.602951
CCTGGGG49350.028.278894
GTACATG211650.027.1320781
TCAACGC216050.027.0122264
ATCAACG216200.026.9079233
CAACGCA217750.026.7584535
AACGCAG225550.026.041956
TACATGG218800.025.8746092
ACATGGG209750.025.7190653
CATGGGG89350.025.4532874
TATCAAC233950.025.1838822
ACGCAGA255500.022.9525017
CGCAGAG258300.022.6127348
TATCACG4800.021.5514282
Produced by FastQC (version 0.11.5)