FastQCFastQC Report
Mon 6 Jun 2016
ERR1211595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11194101
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271200.24227046012895542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233360.20846694165078555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149970.13397234847175313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146800.047.6035461
GTATCAA253000.038.4499551
TCAACGC293850.031.8052144
CAACGCA297450.031.3854585
ATCAACG298300.031.3301893
AACGCAG311250.030.020216
TATCAAC327400.028.8545322
GTACATG329550.027.8015251
TACATGG340800.026.0082022
ACGCAGA359000.025.8839117
CGCAGAG366600.025.0665078
ACATGGG344100.025.0299073
GTACCTG90200.022.1131691
CATGGGG215600.022.0775014
GCAGAGT419750.021.2769249
TACCTGG89950.021.235082
GAGTACT253100.019.54799312-13
TATAACG8800.019.246372
AGAGTAC376850.019.17529910-11
CAGAGTA405900.018.7926310-11
Produced by FastQC (version 0.11.5)