FastQCFastQC Report
Mon 6 Jun 2016
ERR1211595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11194101
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323130.28866096527090473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282660.2525079950591834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189400.16919625792191798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161450.047.717271
GTATCAA279450.037.9274671
TCAACGC340050.030.0963254
ATCAACG343900.029.7734583
CAACGCA345200.029.633455
AACGCAG359300.028.5369536
TATCAAC367700.028.163272
GTACATG346700.027.0892581
TACATGG361000.025.572462
ACATGGG360150.024.8290213
ACGCAGA413400.024.7453787
CGCAGAG424850.023.9678738
GTACCTG81750.023.0692711
CATGGGG226150.022.5648084
TACCTGG83900.021.2440172
GCAGAGT484100.020.7721279
AGAGTAC440200.019.77472710-11
GAGTACT301100.019.49184812-13
ATGGGGG127700.019.2076455
CAGAGTA467850.018.27961510-11
Produced by FastQC (version 0.11.5)