FastQCFastQC Report
Mon 6 Jun 2016
ERR1211594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6294202
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217940.34625517261759314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189450.3009912932568735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124380.197610435762945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74190.11787038293337267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105550.048.48341
TACCTGG66200.039.7999532
GTACCTG67300.039.3860861
GTATCAA183850.038.2760161
ACCTGGG77950.032.915523
TCAACGC217550.031.4062254
CAACGCA220350.030.9617865
ATCAACG220250.030.9368633
AACGCAG229800.029.8278886
TATCAAC232550.029.6599332
TAACGCA6700.027.35274
GTACATG200950.026.921361
ACGCAGA259650.026.2361667
CGCAGAG263950.025.6131658
TACATGG205800.025.4907172
TATAACG7600.025.3818232
ACATGGG202800.024.8863123
CATGGGG121450.023.4082134
GCAGAGT290400.022.4063139
ATAACGC9450.020.3880793
Produced by FastQC (version 0.11.5)