FastQCFastQC Report
Mon 6 Jun 2016
ERR1211594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6294202
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261370.4152551824679284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228210.36257177637451105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158670.2520891448987497No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80560.12799080804842297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119250.048.8558541
GTACCTG68150.040.0469321
TACCTGG66700.039.9729652
GTATCAA203300.036.4942051
ACCTGGG77650.033.585633
TCAACGC246250.029.3477754
ATCAACG247900.029.1529033
CAACGCA250650.028.8508825
TATCAAC257450.028.2374692
AACGCAG261800.027.7486486
GTACATG213650.026.3204981
TATAACG8050.026.2860772
TAACGCA8200.025.2135454
TACATGG222800.024.735552
ACATGGG214650.024.693363
ACGCAGA295250.024.5567597
CGCAGAG300800.024.0568058
CATGGGG126000.023.8300744
ATAACGC8800.023.4948123
CCTGGGG70750.022.6476424
Produced by FastQC (version 0.11.5)