FastQCFastQC Report
Mon 6 Jun 2016
ERR1211593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8013588
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248340.3098986371647756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212650.2653617830115549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140740.17562669805335637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142300.041.0269051
GTATCAA235500.035.0028571
TACCTGG72650.032.634332
GTACCTG76750.031.6433071
TCAACGC274450.029.0036474
ATCAACG276100.028.8813763
CAACGCA277250.028.7417625
GTACATG273700.028.342891
AACGCAG287400.027.8700086
TATCAAC295400.027.3425872
TACATGG281750.026.7805942
CATGGGG169100.025.7873554
ACATGGG280450.025.7693443
ACCTGGG92500.025.5522233
ACGCAGA326150.024.3571077
CGCAGAG336200.023.5180958
ATGGGGG106300.023.2493925
GCAGAGT371350.020.710179
GAGTACT216800.020.0258412-13
AGAGTAC325000.019.28528810-11
Produced by FastQC (version 0.11.5)