FastQCFastQC Report
Mon 6 Jun 2016
ERR1211593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8013588
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290320.36228465950582933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252750.3154017900595838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179470.2239571088506172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167650.038.72491
GTATCAA262600.033.6101951
TACCTGG75000.032.2948462
GTACCTG75250.032.267151
TCAACGC321300.026.7939054
ATCAACG323250.026.6474723
CAACGCA324850.026.5292025
GTACATG283250.026.4492821
TATCAAC337100.025.7828252
ACCTGGG93450.025.6964863
AACGCAG338150.025.6394236
TACATGG291650.025.2693632
CATGGGG169450.024.5427344
ACATGGG289300.024.5116713
ACGCAGA376650.022.9185477
ATGGGGG101450.022.3718995
CGCAGAG386700.022.3229128
TATAACG8700.022.164192
TAACGCA9200.021.452744
GAGTACT253150.020.28856512-13
Produced by FastQC (version 0.11.5)