FastQCFastQC Report
Mon 6 Jun 2016
ERR1211592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893099
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184170.6365838154864386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149110.5153988854166415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130280.45031296889598316No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110180.3808372959238519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108430.3747884189237907No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG56550.1954651396305484No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53200.18388586080185987No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.1798071894532472No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46840.1619025135330661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG65350.070.1172562
TATAACG6600.068.428792
GTACCTG72500.064.171951
ACCTGGG71600.062.612153
TAACGCA8200.056.1610834
ATAACGC9400.049.9914133
GTATAAC10800.049.806661
CCTGGGG46750.046.6401144
GTACCCG7000.046.511181
TATCACG4000.038.8119542
TACCCGG8750.034.947562
CCTGGGA51450.033.154654
CTGGGGG30250.032.3078965
ATCACGC5500.029.0495533
CATGGGG84700.028.7388634
ATGGGGG53000.026.6846395
CCTAGAG13150.025.400612
TCACGCA6500.025.3033454
TGGGGGG64100.024.4809976
GGGTATG9300.023.7440937