FastQCFastQC Report
Mon 6 Jun 2016
ERR1211592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893099
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208140.7194361478815623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173110.5983549128460519No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA171740.5936195062802897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131160.45335468990172817No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113310.3916561445011042No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70450.24351050551674863No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69080.23877509895098648No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56270.19449731931053862No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37660.13017183304131658No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33550.11596561334403005No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32560.11254367721256688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.10034222817815773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG66200.069.76032
GTACCTG70250.066.4545441
ACCTGGG71650.062.436383
TAACGCA6900.057.2067384
TATAACG7050.054.6990282
CCTGGGG45800.046.683444
TATCACG3650.045.095172
GTACCCG7250.042.2776831
ATAACGC9600.040.627853
TACCCGG7750.038.229072
GTATAAC11750.038.126031
CTGGGGG28550.035.2228625
CCTGGGA50350.033.318524
TGGGGGG54050.028.3435276
CATGGGG87750.028.1679734
ATGGGGG50800.027.935745
ATCACGC6350.025.9006843
GTACACG6950.025.7830681
ACAACGC4850.025.1911833
ACCCGGG11250.025.0623