##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2893099 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03320073042782 33.0 31.0 34.0 30.0 34.0 2 32.26225925901603 34.0 31.0 34.0 30.0 34.0 3 32.34643474004865 34.0 31.0 34.0 30.0 34.0 4 36.00300162559249 37.0 35.0 37.0 35.0 37.0 5 35.923848440720484 37.0 35.0 37.0 35.0 37.0 6 35.96718708900041 37.0 35.0 37.0 35.0 37.0 7 35.88453557932169 37.0 35.0 37.0 35.0 37.0 8 35.86508308218972 37.0 35.0 37.0 35.0 37.0 9 37.49432563489877 39.0 37.0 39.0 35.0 39.0 10-11 37.44200613252433 39.0 37.0 39.0 34.5 39.0 12-13 37.34053328282233 39.0 37.0 39.0 34.0 39.0 14-15 38.515987354736225 40.0 38.0 41.0 34.0 41.0 16-17 38.43940338716373 40.0 38.0 41.0 34.0 41.0 18-19 38.46971724783701 40.0 38.0 41.0 34.0 41.0 20-21 38.40819377421927 40.0 38.0 41.0 34.0 41.0 22-23 38.27175668720635 40.0 38.0 41.0 34.0 41.0 24-25 38.160204852996735 40.0 37.0 41.0 33.5 41.0 26-27 37.93089953022693 40.0 37.0 41.0 33.0 41.0 28-29 37.91430331281439 40.0 37.0 41.0 33.0 41.0 30-31 37.71826111031804 40.0 37.0 41.0 33.0 41.0 32-33 37.384733464012115 39.5 36.0 41.0 32.0 41.0 34-35 37.17461535191157 39.0 36.0 41.0 31.5 41.0 36-37 37.026147739845754 39.0 36.0 41.0 31.0 41.0 38-39 36.918097686943995 39.0 35.0 41.0 31.0 41.0 40-41 36.746382685141434 39.0 35.0 41.0 30.0 41.0 42-43 36.53551174709196 39.0 35.0 41.0 30.0 41.0 44-45 36.28794935811045 39.0 35.0 41.0 29.5 41.0 46-47 35.941649248781324 39.0 35.0 40.5 27.5 41.0 48-49 35.61610058971366 39.0 35.0 40.0 26.0 41.0 50-51 35.38052085324422 38.5 34.5 40.0 24.5 41.0 52-53 35.19013573334338 38.0 34.0 40.0 24.0 41.0 54-55 34.97761276057266 38.0 34.0 40.0 24.0 41.0 56-57 34.74575083673251 38.0 34.0 40.0 23.0 41.0 58-59 34.483501428744745 38.0 33.0 40.0 23.0 41.0 60-61 34.13704957901545 37.0 33.0 40.0 22.0 41.0 62-63 33.73141776344329 36.5 32.0 40.0 22.0 41.0 64-65 33.44741642093824 36.0 32.0 39.0 21.5 41.0 66-67 33.06680621713947 36.0 31.0 39.0 20.0 40.0 68-69 32.662841299243475 35.0 31.0 38.5 20.0 40.0 70-71 32.28010672984229 35.0 31.0 38.0 19.0 40.0 72-73 31.858797262036315 35.0 30.0 37.0 18.0 39.5 74-75 31.458317534242692 34.5 30.0 37.0 18.0 39.0 76-77 29.959568442006304 33.0 28.0 35.0 16.0 37.5 78-79 30.819495461441175 34.0 29.0 36.0 18.0 38.0 80-81 30.792439698745184 34.0 29.5 35.0 18.0 37.0 82-83 30.53996631293986 34.0 29.0 35.0 17.0 37.0 84-85 30.228339576350482 34.0 29.0 35.0 15.5 36.5 86-87 29.930493736992755 34.0 29.0 35.0 14.5 36.0 88-89 29.662979386464134 34.0 29.0 35.0 12.5 36.0 90-91 29.368997742559102 34.0 27.5 35.0 11.0 35.0 92-93 29.105704125576068 33.5 27.0 35.0 10.0 35.0 94-95 28.933425886912268 33.0 27.0 35.0 10.0 35.0 96-97 28.69802467872686 33.0 26.0 35.0 10.0 35.0 98-99 28.451341105160935 33.0 25.0 35.0 10.0 35.0 100 26.775480894362758 31.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2822721422571206 1101 2 0.18533806496759553 1101 3 0.16004266391443167 1101 4 0.12684261216782744 1101 5 0.10554973631610665 1101 6 0.07729906365239714 1101 7 0.18555604905294132 1101 8 0.12415089680182234 1101 9 0.10560968849928543 1101 10-11 0.05654728071526449 1101 12-13 0.07230924588128573 1101 14-15 0.09431568375664057 1101 16-17 0.10515869469733019 1101 18-19 0.017814131659484644 1101 20-21 0.06534281941797815 1101 22-23 -0.044844674771404414 1101 24-25 -0.05851420079221015 1101 26-27 -0.1493772771891102 1101 28-29 0.06031398272939015 1101 30-31 0.13290710670298012 1101 32-33 0.1435462662334004 1101 34-35 0.17986598633358852 1101 36-37 0.05580830214150012 1101 38-39 0.0688518122891324 1101 40-41 0.06790560194292539 1101 42-43 0.09497314402562296 1101 44-45 0.1194922945791248 1101 46-47 0.04925844741546115 1101 48-49 -0.11494623980258467 1101 50-51 -0.047455736915416935 1101 52-53 -0.08772750545097097 1101 54-55 -0.2692040604464978 1101 56-57 -0.2792844515507227 1101 58-59 -0.31081607725931093 1101 60-61 -0.25029937777274824 1101 62-63 -0.3393914552134376 1101 64-65 -0.2796239933972515 1101 66-67 -0.311463732194575 1101 68-69 -0.27146227778428056 1101 70-71 -0.41600340212184506 1101 72-73 -0.5316752584376623 1101 74-75 -0.6163309736137741 1101 76-77 -0.3930179795770634 1101 78-79 -0.31472958751083624 1101 80-81 -0.5263633827038987 1101 82-83 -0.5239672455770936 1101 84-85 -0.5468784139966942 1101 86-87 -0.7043413622832517 1101 88-89 -0.6273864213647933 1101 90-91 -0.7365334360586395 1101 92-93 -0.8021275051372676 1101 94-95 -0.8633142287821371 1101 96-97 -1.0804616318684381 1101 98-99 -1.0894658314086811 1101 100 -1.1182335478086216 1102 1 0.21979041711092862 1102 2 0.173369959396652 1102 3 0.11432763621360209 1102 4 0.07003474820968592 1102 5 0.0279008443493467 1102 6 0.11527228618241026 1102 7 0.09082690162296103 1102 8 0.12937077214807857 1102 9 0.08316143519057562 1102 10-11 0.14709004387316327 1102 12-13 0.15703291085512916 1102 14-15 0.12291679178988346 1102 16-17 0.12666386936646035 1102 18-19 0.05505684095464858 1102 20-21 -0.009732684583255491 1102 22-23 0.004023246127339064 1102 24-25 -0.026780398083680268 1102 26-27 -0.012866042963196378 1102 28-29 -0.0225696930169903 1102 30-31 -0.014200176307177514 1102 32-33 0.017252118033958652 1102 34-35 0.0026249121569108524 1102 36-37 -0.09836269278001453 1102 38-39 -0.04732720433135995 1102 40-41 -0.210185733852704 1102 42-43 -0.1453796568361838 1102 44-45 -0.07782563016695576 1102 46-47 -0.2795383939203262 1102 48-49 -0.2983531534683337 1102 50-51 -0.17394850392064853 1102 52-53 -0.3735385242259781 1102 54-55 -0.29267907198851617 1102 56-57 -0.3749735097224658 1102 58-59 -0.26199720991551345 1102 60-61 -0.32220467555668364 1102 62-63 -0.3259748270201399 1102 64-65 -0.26955118253237487 1102 66-67 -0.4685562991382426 1102 68-69 -0.46614862358298836 1102 70-71 -0.4925245432421903 1102 72-73 -0.510960711654036 1102 74-75 -0.6112149763838559 1102 76-77 -0.5510916176195373 1102 78-79 -0.4070161564551249 1102 80-81 -0.35049340347952196 1102 82-83 -0.4884779604124283 1102 84-85 -0.6728517904202072 1102 86-87 -0.6561390307966413 1102 88-89 -0.5341365752583016 1102 90-91 -0.7200730828730393 1102 92-93 -0.831152301228375 1102 94-95 -0.7984048335836178 1102 96-97 -0.8938171835766546 1102 98-99 -1.1200574557546332 1102 100 -0.9919033308187792 1103 1 0.33484710827818986 1103 2 0.28181593618744216 1103 3 0.21858942742105825 1103 4 0.1882234310601092 1103 5 0.1317241331900476 1103 6 0.1667910786031328 1103 7 0.14119655194114955 1103 8 0.2075999708758971 1103 9 0.21942531031375978 1103 10-11 0.27584784561502573 1103 12-13 0.276648461817409 1103 14-15 0.2798109267060127 1103 16-17 0.2763801311567349 1103 18-19 0.22642726026973747 1103 20-21 0.23963191642782533 1103 22-23 0.26683664531014983 1103 24-25 0.14426676251054715 1103 26-27 0.19558959335115134 1103 28-29 0.25383727953537516 1103 30-31 0.3796244232167467 1103 32-33 0.4869474512486889 1103 34-35 0.3556666149438499 1103 36-37 0.34382276442487836 1103 38-39 0.4024580389286214 1103 40-41 0.30246430054979356 1103 42-43 0.25128685452511235 1103 44-45 0.26631048605040775 1103 46-47 0.38088805580826346 1103 48-49 0.3327564657699611 1103 50-51 0.12120294470197734 1103 52-53 0.18724276298259923 1103 54-55 0.21086994991707542 1103 56-57 0.15893547709351452 1103 58-59 0.18565011370229456 1103 60-61 0.18432356225443414 1103 62-63 0.0684863480485447 1103 64-65 0.05316514608626477 1103 66-67 0.053574069368458765 1103 68-69 -0.0017125538631077575 1103 70-71 -0.0147360941028154 1103 72-73 -0.027963590921519454 1103 74-75 0.03828697575761453 1103 76-77 -0.014346131123101458 1103 78-79 0.1466411438856987 1103 80-81 0.16818671498553428 1103 82-83 0.1817566118812799 1103 84-85 0.1611321194725548 1103 86-87 0.004252122256335866 1103 88-89 -0.005922354086507653 1103 90-91 -0.12612898057103905 1103 92-93 -0.19060811590859217 1103 94-95 -0.17686014281543905 1103 96-97 -0.24004209822929212 1103 98-99 -0.4773125227100721 1103 100 -0.5017272192323396 1104 1 0.17324648600005332 1104 2 0.026342110000413754 1104 3 0.0873317847562518 1104 4 0.08144065471138617 1104 5 0.12216124344441681 1104 6 0.118796008662585 1104 7 0.08617836179337957 1104 8 0.1033154419477782 1104 9 0.1721907871641406 1104 10-11 0.11540569042334425 1104 12-13 0.09203974203426668 1104 14-15 0.11521690575256827 1104 16-17 0.10575519560251934 1104 18-19 0.1235404696318696 1104 20-21 0.015105562685675977 1104 22-23 0.0677069488991151 1104 24-25 0.0419489978952754 1104 26-27 0.11297563070859695 1104 28-29 0.10413222497876973 1104 30-31 0.17873937927596018 1104 32-33 0.1493305924384245 1104 34-35 0.20305137895635283 1104 36-37 0.24041348713810606 1104 38-39 0.2868741306869609 1104 40-41 0.2457130810105852 1104 42-43 0.20312119788395933 1104 44-45 0.26658262893078444 1104 46-47 0.31401114881850134 1104 48-49 0.11452486508540005 1104 50-51 0.09996732260030683 1104 52-53 0.06668242892332898 1104 54-55 0.03686667105384345 1104 56-57 0.23114918728158784 1104 58-59 0.04713821104120797 1104 60-61 -0.022563676822315415 1104 62-63 -0.07355147444855703 1104 64-65 -0.11639595820829385 1104 66-67 0.022035079753834452 1104 68-69 -0.12578911926424752 1104 70-71 0.08223396775822067 1104 72-73 0.07301577743018228 1104 74-75 0.03353961362315516 1104 76-77 0.036288707456204605 1104 78-79 0.007505717422407798 1104 80-81 0.009881737798476564 1104 82-83 0.09770373484834138 1104 84-85 -0.1776715798713262 1104 86-87 -0.12840152861047116 1104 88-89 -0.13387120298959942 1104 90-91 -0.0024671173213661746 1104 92-93 -0.18456803184014348 1104 94-95 -0.16693566462735276 1104 96-97 -0.35320751611827106 1104 98-99 -0.16363454887869722 1104 100 -0.06207477319897947 1105 1 0.33048816986168816 1105 2 0.2423133913359763 1105 3 0.18071155954948637 1105 4 0.15304848862189857 1105 5 0.16258329391374104 1105 6 0.1249005295751644 1105 7 0.18520780123176905 1105 8 0.138776963424732 1105 9 0.15915814691010866 1105 10-11 0.19430214205429053 1105 12-13 0.22237015506336633 1105 14-15 0.25979402310682786 1105 16-17 0.1950159199279824 1105 18-19 0.2802653017539214 1105 20-21 0.18457422416945946 1105 22-23 0.18652235746431245 1105 24-25 0.23101591535117194 1105 26-27 0.11117909884560362 1105 28-29 0.17444405830463694 1105 30-31 0.36743064761754596 1105 32-33 0.3247219103095418 1105 34-35 0.3383964625761493 1105 36-37 0.3076726700990591 1105 38-39 0.2173513610255995 1105 40-41 0.21383442993402468 1105 42-43 0.35694416287840625 1105 44-45 0.334615947625835 1105 46-47 0.2550796900767338 1105 48-49 0.17558867842299009 1105 50-51 0.3714212094434757 1105 52-53 0.24694548802166594 1105 54-55 0.2787158539501746 1105 56-57 0.2486549150393671 1105 58-59 0.2142759987352889 1105 60-61 0.1400789656798267 1105 62-63 0.2996299638508546 1105 64-65 0.33838455553976843 1105 66-67 0.18769049053817355 1105 68-69 0.22166843138320758 1105 70-71 0.21101332425747898 1105 72-73 0.20926021315552745 1105 74-75 0.1592238754180606 1105 76-77 0.13347187383066128 1105 78-79 0.3365691056481914 1105 80-81 0.1337690221205179 1105 82-83 0.28842364209019067 1105 84-85 0.1748579022342085 1105 86-87 0.1378309246656606 1105 88-89 0.12152758330281799 1105 90-91 0.10787346006869925 1105 92-93 0.2940308860854266 1105 94-95 0.14659476640438385 1105 96-97 0.29867648896079046 1105 98-99 0.24497304061480207 1105 100 0.24485138545907503 1106 1 0.2272133887872485 1106 2 0.17604371617924386 1106 3 0.1589433222533927 1106 4 0.06712742348150158 1106 5 0.10333417250787136 1106 6 0.09775258604717862 1106 7 0.14506914080776312 1106 8 0.1375772134797444 1106 9 0.19355256680973554 1106 10-11 0.23999490900695974 1106 12-13 0.19350773695338574 1106 14-15 0.16986429955677806 1106 16-17 0.17520298252838273 1106 18-19 0.19829478005771506 1106 20-21 0.24929451778244527 1106 22-23 0.294904884460351 1106 24-25 0.22871031905174277 1106 26-27 0.23579509133065102 1106 28-29 0.21151331782579774 1106 30-31 0.4039503910829083 1106 32-33 0.3338115008721516 1106 34-35 0.2511218007232614 1106 36-37 0.1815190409441101 1106 38-39 0.21748238284956756 1106 40-41 0.32029090841752605 1106 42-43 0.3266836061758127 1106 44-45 0.3514706172214801 1106 46-47 0.3105112842749449 1106 48-49 0.27606103287353534 1106 50-51 0.1997374133584202 1106 52-53 0.23352408881716258 1106 54-55 0.16786302541891018 1106 56-57 0.2781218617510959 1106 58-59 0.19020050312931858 1106 60-61 0.19267306483359903 1106 62-63 0.19222874322606032 1106 64-65 0.15911683956095857 1106 66-67 0.3166999522528826 1106 68-69 0.25643940745091953 1106 70-71 0.2566236000913733 1106 72-73 0.08718598865514338 1106 74-75 0.08423241261174752 1106 76-77 0.22335749870337906 1106 78-79 0.1882271937555764 1106 80-81 0.2908557682076456 1106 82-83 0.262976987861272 1106 84-85 0.17106929400840798 1106 86-87 0.145130419170723 1106 88-89 0.1962148427960564 1106 90-91 0.19025062032218543 1106 92-93 0.22781273325270845 1106 94-95 0.13261687070019335 1106 96-97 0.3241801501482193 1106 98-99 0.31306682764301286 1106 100 0.15527323251998482 1107 1 0.31173913939895925 1107 2 0.19322815964427065 1107 3 0.17846107227456542 1107 4 0.1675156203850463 1107 5 0.07076275882414507 1107 6 0.13080924586033404 1107 7 0.14388045062493404 1107 8 0.12709502863462063 1107 9 0.2628834916432652 1107 10-11 0.12540278714613606 1107 12-13 0.1483883142985789 1107 14-15 0.12490250047689955 1107 16-17 0.13705568915964506 1107 18-19 0.10306723544655227 1107 20-21 0.09451674314072989 1107 22-23 0.14124968928719284 1107 24-25 0.09919725865759688 1107 26-27 0.18636316582624346 1107 28-29 0.17519932124314863 1107 30-31 0.23360880820352037 1107 32-33 0.2056594093202122 1107 34-35 0.16103113460082596 1107 36-37 0.25807752775779136 1107 38-39 0.28421555826984246 1107 40-41 0.2794655340615364 1107 42-43 0.3111329332360526 1107 44-45 0.32562339050623734 1107 46-47 0.38498940043439944 1107 48-49 0.3248765763817616 1107 50-51 0.3252711298227027 1107 52-53 0.31612654956867914 1107 54-55 0.21982159603795992 1107 56-57 0.24075172208271312 1107 58-59 0.1646037155238247 1107 60-61 0.18798940840088108 1107 62-63 0.19082804282157895 1107 64-65 0.4134533333265793 1107 66-67 0.37269355612269095 1107 68-69 0.3873617441878494 1107 70-71 0.30279069844979034 1107 72-73 0.27798834910074177 1107 74-75 0.25172569841194914 1107 76-77 0.2536527063822085 1107 78-79 0.378460502699955 1107 80-81 0.47941937453082417 1107 82-83 0.47998784999274946 1107 84-85 0.3967889672108811 1107 86-87 0.4680025551872724 1107 88-89 0.2962179909253102 1107 90-91 0.2092312343593683 1107 92-93 0.35661724745294165 1107 94-95 0.37669447968517034 1107 96-97 0.5108851660951181 1107 98-99 0.4779304485127227 1107 100 0.570415299994167 1108 1 0.21647578283651114 1108 2 0.1133138760380632 1108 3 0.07228181423036517 1108 4 0.019145525739858726 1108 5 0.024416131394254137 1108 6 0.06090675681280544 1108 7 0.047154910967734054 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72-73 -0.041827507052691004 2316 74-75 0.2048502352291237 2316 76-77 0.2070270972495294 2316 78-79 -0.00849314322318051 2316 80-81 0.07707666511673494 2316 82-83 0.13430549535527447 2316 84-85 0.29017343088676384 2316 86-87 0.46712206669134915 2316 88-89 0.43156296193195587 2316 90-91 0.17545259012512204 2316 92-93 0.3505067127471264 2316 94-95 0.42755379410904837 2316 96-97 0.5194134269274251 2316 98-99 0.4641473375575593 2316 100 0.3467270589646603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 29.0 12 48.0 13 183.0 14 570.0 15 1510.0 16 3701.0 17 8391.0 18 15867.0 19 22125.0 20 26873.0 21 32098.0 22 39074.0 23 41931.0 24 41281.0 25 41103.0 26 44614.0 27 50771.0 28 59209.0 29 69375.0 30 80659.0 31 97399.0 32 116939.0 33 146841.0 34 194818.0 35 259384.0 36 367853.0 37 548019.0 38 506266.0 39 76145.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.55300177637128 18.07345153167839 12.514615384082244 21.858931307868083 2 15.44816783232266 21.406903022057 41.1610272717492 21.983901873871144 3 17.347153575930736 31.165610284782396 30.232650582378255 21.254585556908605 4 11.215429433390435 17.43007768915214 37.86319574928761 33.49129712816982 5 11.087000028689038 37.91097480169134 33.95098875216853 17.051036417451094 6 27.063233647185697 36.23218161958881 22.103709541388415 14.600875191837073 7 26.594216098377554 32.79504088868027 23.793620612360655 16.81712240058152 8 28.24720573839849 32.8773013599385 21.73091440517208 17.14457849649093 9 29.373381277308518 15.120706204661507 21.302658498724032 34.20325401930594 10-11 24.68016851210092 26.38998633293307 29.304279236773468 19.625565918192546 12-13 25.68052605964074 24.47558913481108 29.29834403481993 20.545540770728255 14-15 23.424379877771205 24.94899068438377 28.344156214495253 23.28247322334977 16-17 21.62710438452364 27.050048278721434 29.433273311139118 21.889574025615808 18-19 21.542617633974785 25.78986354600643 31.954834720156455 20.712684099862326 20-21 22.08260054419963 25.808176544111138 31.92041564352712 20.18880726816211 22-23 21.61661623342586 25.76462571184493 31.208736930318825 21.410021124410385 24-25 20.99803705300095 26.069657484932247 31.313705476376715 21.618599985690086 26-27 21.407157143620044 25.38882660458322 31.46392851532208 21.74008773647465 28-29 21.62772337734566 25.745328550842313 31.665406751967556 20.961541319844468 30-31 22.53309960064617 25.856497048010084 31.6430574876294 19.967345863714346 32-33 21.54420243566558 25.957169838451883 31.918802160242905 20.579825565639638 34-35 21.566352496975856 26.296734203385135 31.40937019902906 20.727543100609942 36-37 22.082087676928143 26.04025920022896 31.21883698639465 20.658816136448245 38-39 22.585496143098712 26.396009534752206 30.425448220460726 20.593046101688355 40-41 21.84073790046391 26.56513224311641 30.844040975342995 20.750088881076685 42-43 21.8513339498224 26.355875515301314 30.439066969725776 21.353723565150506 44-45 21.76731617743646 26.651005031726566 30.417972763135516 21.16370602770146 46-47 21.590791640064115 26.075489138381542 30.647141114448406 21.68657810710593 48-49 21.630723080853105 26.433511827006207 30.634761570776586 21.301003521364105 50-51 21.328438667171344 27.03705047970148 30.365233352172265 21.269277500954907 52-53 21.36361405269831 27.333204055588045 29.03127069653743 22.271911195176212 54-55 21.16832539128508 27.287697910997323 29.494176416999274 22.049800280718323 56-57 21.82142225073557 26.91886116518815 29.018590442006058 22.241126142070218 58-59 21.7884157755501 27.15922471346382 29.566946124094446 21.48541338689164 60-61 22.45835634266929 27.54663009370525 29.264971623979484 20.73004193964597 62-63 21.679217813692688 27.417467760499605 29.79854730606218 21.104767119745528 64-65 21.358864367117587 27.06065582242419 29.510038727955944 22.07044108250228 66-67 21.7652509356214 27.00946657178951 29.33531084339099 21.889971649198102 68-69 21.878202605245097 26.979883080115236 29.249036658070786 21.89287765656888 70-71 21.382869507806856 27.30681617852933 29.382053737162845 21.928260576500964 72-73 21.552213028457803 27.595950733591184 28.612692234630956 22.239144003320057 74-75 21.90792273233379 27.367308328490942 28.671850937465543 22.05291800170973 76-77 21.374306897649507 26.998576895516752 29.670000148636127 21.95711605819761 78-79 21.430556765524596 27.189680969192114 29.387624298390808 21.992137966892482 80-81 21.131522789481714 27.69482922903795 28.8714759061289 22.302172075351432 82-83 21.377652049941435 27.824382196643377 28.456411174735575 22.341554578679613 84-85 21.088924457142838 28.276076185056414 27.952539141741756 22.682460216058992 86-87 21.203758296269445 27.6033610121963 28.47583375811823 22.717046933416025 88-89 21.080422835027445 27.508631887900837 28.856770727550398 22.554174549521324 90-91 21.896017708294437 27.74882062261168 28.576372897624097 21.778788771469788 92-93 21.089981892121994 27.895397833105008 28.737435768893345 22.277184505879656 94-95 20.721910457628088 27.609661546451118 28.588353236958973 23.080074758961818 96-97 21.29871773131734 27.44740530777404 28.679856674094324 22.574020286814296 98-99 21.556639983875893 27.151158479321197 28.415573244978027 22.876628291824883 100 19.987809971070288 28.368748720842113 28.826929246665895 22.816512061421705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1291.0 1 1173.0 2 4021.0 3 6118.0 4 3765.5 5 2541.0 6 3332.0 7 5815.5 8 8947.5 9 10137.5 10 8777.5 11 7139.5 12 7096.0 13 7556.5 14 7898.0 15 8328.0 16 8763.5 17 9051.5 18 9375.0 19 9558.0 20 9821.5 21 10282.5 22 11022.0 23 12175.0 24 13563.5 25 15350.0 26 18095.5 27 21166.0 28 23912.0 29 27710.5 30 33175.0 31 38214.0 32 44291.5 33 51723.5 34 59335.5 35 65473.5 36 73785.5 37 82146.5 38 85416.5 39 88164.5 40 87075.0 41 84685.5 42 83986.0 43 83484.0 44 85118.5 45 88636.0 46 90984.0 47 91466.5 48 95494.5 49 104950.5 50 128569.0 51 130541.5 52 104291.5 53 91486.0 54 87395.0 55 81742.0 56 75473.5 57 71434.0 58 65795.5 59 56805.0 60 44626.5 61 33783.0 62 27181.5 63 21112.0 64 15447.5 65 11452.5 66 8627.0 67 6609.5 68 4930.5 69 3631.5 70 2833.0 71 2106.5 72 1589.0 73 1325.5 74 1125.0 75 832.5 76 538.5 77 356.5 78 238.0 79 153.0 80 97.5 81 64.0 82 41.0 83 33.5 84 29.0 85 19.5 86 12.5 87 5.5 88 4.0 89 4.5 90 4.0 91 3.5 92 1.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25719825004260133 2 0.08475340802371435 3 0.0016591205485882094 4 0.0010715153542965518 5 2.7652009143136827E-4 6 6.567352171494995E-4 7 0.0 8 9.332553085808679E-4 9 0.0 10-11 4.493451485759734E-4 12-13 0.0014862954914436042 14-15 0.0 16-17 8.641252857230257E-5 18-19 0.001244340411441157 20-21 0.0014862954914436042 22-23 1.7282505714460517E-5 24-25 0.0 26-27 1.209775400012236E-4 28-29 0.0015899905257303673 30-31 0.0017455330771605118 32-33 0.0031454160400318136 34-35 0.004476168980045273 36-37 0.0015899905257303673 38-39 0.003560196177178866 40-41 0.006515504654351614 42-43 0.012927314274416464 44-45 0.008814077914374862 46-47 0.007120392354357732 48-49 0.006429092125779311 50-51 0.005323011760053838 52-53 0.003560196177178866 54-55 0.005651379368628588 56-57 0.0036984562228945497 58-59 0.001814663100018354 60-61 0.008693100374373638 62-63 0.011596561334403005 64-65 0.007863540100079534 66-67 0.008140060191510902 68-69 0.015018497465866186 70-71 0.004458886474330813 72-73 0.013048291814417688 74-75 0.008502992811514573 76-77 0.004424321462901892 78-79 0.016003600291590436 80-81 0.00603159449434672 82-83 0.014897519925864963 84-85 0.024005400437385655 86-87 0.015848057740160293 88-89 0.016643053003025477 90-91 0.030935685228884322 92-93 0.024593005631677312 94-95 0.030088842448875754 96-97 0.018336738563042607 98-99 0.01850956362018721 100 0.01973662152591391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2893099.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.79888463125968 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01084465882495 42.41320351842375 2 15.27917958325727 17.051223576529413 3 4.229666623055025 7.080320397856112 4 1.551697571571498 3.4633197511489535 5 0.798306378549948 2.227230275855364 6 0.47158139392188453 1.5788229476217517 7 0.2953087503125133 1.153452922250258 8 0.22511762975385916 1.0049050120878578 9 0.16417001129442862 0.824445316811738 >10 0.8601317728559188 8.72466113710324 >50 0.06375090518008882 2.4647910317065875 >100 0.04396178412126517 4.655175746687596 >500 0.003725927934565059 1.441825072873399 >1k 0.0019725500830037397 1.867577032528963 >5k 2.1917223144485998E-4 0.7981830113050247 >10k+ 3.6528705240809994E-4 3.2508632492099334 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20814 0.7194361478815623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17311 0.5983549128460519 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 17174 0.5936195062802897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13116 0.45335468990172817 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11331 0.3916561445011042 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7045 0.24351050551674863 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6908 0.23877509895098648 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5627 0.19449731931053862 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 3766 0.13017183304131658 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3355 0.11596561334403005 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3256 0.11254367721256688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.10034222817815773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.456501142892103E-5 3.456501142892103E-5 5 0.0 0.0 0.0 3.456501142892103E-5 3.456501142892103E-5 6 0.0 0.0 0.0 3.456501142892103E-5 3.456501142892103E-5 7 0.0 0.0 0.0 6.913002285784205E-5 3.456501142892103E-5 8 0.0 0.0 0.0 6.913002285784205E-5 3.456501142892103E-5 9 0.0 0.0 0.0 6.913002285784205E-5 3.456501142892103E-5 10-11 0.0 0.0 0.0 8.641252857230257E-5 3.456501142892103E-5 12-13 0.0 0.0 0.0 1.0369503428676309E-4 3.456501142892103E-5 14-15 0.0 0.0 0.0 1.0369503428676309E-4 3.456501142892103E-5 16-17 0.0 0.0 0.0 1.0369503428676309E-4 6.913002285784205E-5 18-19 0.0 0.0 0.0 1.382600457156841E-4 1.0369503428676309E-4 20-21 0.0 0.0 0.0 1.7282505714460514E-4 1.0369503428676309E-4 22-23 0.0 0.0 0.0 5.70322688577197E-4 1.0369503428676309E-4 24-25 0.0 0.0 0.0 8.986902971519468E-4 1.0369503428676309E-4 26-27 0.0 0.0 0.0 0.001710968065731591 1.0369503428676309E-4 28-29 0.0 0.0 0.0 0.0033355236028908795 1.0369503428676309E-4 30-31 0.0 0.0 0.0 0.009349835591523139 1.0369503428676309E-4 32-33 0.0 0.0 0.0 0.020324226720205563 1.0369503428676309E-4 34-35 0.0 0.0 0.0 0.03599945940322125 1.0369503428676309E-4 36-37 0.0 0.0 0.0 0.0582593267634464 1.0369503428676309E-4 38-39 0.0 0.0 0.0 0.09807821992956342 1.382600457156841E-4 40-41 0.0 0.0 0.0 0.15981133034161638 1.382600457156841E-4 42-43 0.0 0.0 0.0 0.21012070447641096 1.5554255143014462E-4 44-45 0.0 0.0 0.0 0.26065475118549347 1.7282505714460514E-4 46-47 0.0 0.0 0.0 0.31784256259464333 1.7282505714460514E-4 48-49 0.0 0.0 0.0 0.3807508833952796 2.0739006857352618E-4 50-51 0.0 0.0 0.0 0.45496196293317304 2.0739006857352618E-4 52-53 0.0 0.0 0.0 0.5317999833396645 2.765200914313682E-4 54-55 0.0 0.0 0.0 0.6237429137405944 3.456501142892103E-4 56-57 0.0 0.0 0.0 0.7122120604929179 3.456501142892103E-4 58-59 0.0 0.0 0.0 0.8144553642996661 3.802151257181313E-4 60-61 0.0 0.0 0.0 0.9133285794920948 3.802151257181313E-4 62-63 0.0 0.0 0.0 1.0254229115560856 4.1478013714705235E-4 64-65 0.0 0.0 0.0 1.1301894611971455 4.1478013714705235E-4 66-67 0.0 0.0 0.0 1.2406765202296914 4.1478013714705235E-4 68-69 0.0 0.0 0.0 1.3823930670882676 4.1478013714705235E-4 70-71 0.0 0.0 0.0 1.5443128631270482 4.1478013714705235E-4 72-73 0.0 0.0 0.0 1.6773363096112508 4.493451485759734E-4 74-75 0.0 0.0 0.0 1.8048120717611114 4.493451485759734E-4 76-77 0.0 0.0 0.0 1.9467705736996903 4.493451485759734E-4 78-79 0.0 0.0 0.0 2.1129764311556567 4.493451485759734E-4 80-81 0.0 0.0 0.0 2.2668944270486424 4.666276542904339E-4 82-83 0.0 0.0 0.0 2.4367296107046457 4.839101600048944E-4 84-85 0.0 0.0 0.0 2.6089497801492447 4.839101600048944E-4 86-87 0.0 0.0 0.0 2.7860436162053217 4.839101600048944E-4 88 0.0 0.0 0.0 2.92641212761817 4.839101600048944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6620 0.0 69.7603 2 GTACCTG 7025 0.0 66.454544 1 ACCTGGG 7165 0.0 62.43638 3 TAACGCA 690 0.0 57.206738 4 TATAACG 705 0.0 54.699028 2 CCTGGGG 4580 0.0 46.68344 4 TATCACG 365 0.0 45.09517 2 GTACCCG 725 0.0 42.277683 1 ATAACGC 960 0.0 40.62785 3 TACCCGG 775 0.0 38.22907 2 GTATAAC 1175 0.0 38.12603 1 CTGGGGG 2855 0.0 35.222862 5 CCTGGGA 5035 0.0 33.31852 4 TGGGGGG 5405 0.0 28.343527 6 CATGGGG 8775 0.0 28.167973 4 ATGGGGG 5080 0.0 27.93574 5 ATCACGC 635 0.0 25.900684 3 GTACACG 695 0.0 25.783068 1 ACAACGC 485 0.0 25.191183 3 ACCCGGG 1125 0.0 25.062 3 >>END_MODULE