FastQCFastQC Report
Mon 6 Jun 2016
ERR1211591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9302935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280640.3016682369596262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233730.2512432904239361No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155650.16731278892091583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140500.049.4279061
GTATCAA242250.038.5799261
GTACCTG85950.032.824341
TACCTGG82350.032.7950972
TCAACGC291350.031.2714924
CAACGCA294050.031.0445985
ATCAACG295350.030.8330613
AACGCAG305750.029.9443196
GTACATG315000.029.355281
TATCAAC314700.029.1390722
TACATGG322700.028.227152
ACATGGG327100.026.8059313
ACGCAGA350750.025.9290187
CATGGGG201350.025.4600324
CGCAGAG361300.025.0038038
ACCTGGG106900.024.7474523
TAACGCA8550.024.1808954
TATAACG9050.023.395082
GCAGAGT402050.021.7567679
ATGGGGG122500.021.09415
Produced by FastQC (version 0.11.5)