FastQCFastQC Report
Mon 6 Jun 2016
ERR1211591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9302935
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325430.3498143327885232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278910.29980860878851673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195250.20988000023648448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161950.046.4127161
GTATCAA271500.036.143641
TACCTGG83250.031.4692882
GTACCTG86100.030.9986571
TCAACGC336750.028.604534
CAACGCA339950.028.2938085
ATCAACG340650.028.2771993
AACGCAG353250.027.3755876
GTACATG345900.027.2379551
TATCAAC358400.026.981812
TACATGG357250.025.949582
ACATGGG358300.024.877773
ACCTGGG104800.024.6151223
CATGGGG220650.023.8933754
ACGCAGA404900.023.7903967
CGCAGAG417200.022.9425838
TATAACG9450.021.4019132
ATGGGGG123500.021.3824735
GCAGAGT460750.020.539169
AGAGTAC421350.019.55470810-11
Produced by FastQC (version 0.11.5)