FastQCFastQC Report
Mon 6 Jun 2016
ERR1211590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10991316
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284600.2589316875249515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246040.22384944623555542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156600.1424761147800682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149000.048.1958541
GTATCAA261700.038.4671631
TCAACGC304900.031.8720654
CAACGCA309000.031.413895
ATCAACG310850.031.2299163
AACGCAG322950.030.1409476
TATCAAC336950.029.0409832
GTACATG323950.027.844091
TACATGG334850.026.1462572
ACGCAGA370700.026.0690987
CGCAGAG379800.025.2718818
ACATGGG339050.024.9736713
GTACCTG89050.023.6154921
TACCTGG87700.022.6373862
CATGGGG198550.022.3181844
GCAGAGT433700.021.4267889
GAGTACT268500.019.63411312-13
AGAGTAC389000.019.53044910-11
CAGAGTA420600.018.87299210-11
GAGTACA240150.018.1027411
Produced by FastQC (version 0.11.5)