FastQCFastQC Report
Mon 6 Jun 2016
ERR1211590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10991316
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341200.3104268860980796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298310.271405171136923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199790.1817707724898456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169050.046.7921371
GTATCAA288000.037.4343071
TCAACGC343500.030.5050144
ATCAACG347100.030.18893
CAACGCA348750.030.0049675
AACGCAG364300.028.866366
TATCAAC374250.028.2837432
GTACATG343950.026.876851
TACATGG359550.025.1895332
ACGCAGA421100.024.8165077
ACATGGG355350.024.3970363
GTACCTG90100.024.015081
CGCAGAG434500.023.964658
TACCTGG92250.022.5819022
CATGGGG207100.022.2804784
GCAGAGT488700.021.047029
GAGTACT304550.020.16532112-13
AGAGTAC445650.019.53221510-11
TATAACG9950.018.9042782
TAACGCA10950.018.452174
Produced by FastQC (version 0.11.5)