FastQCFastQC Report
Mon 6 Jun 2016
ERR1211589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7391767
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259990.3517291602941489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219790.2973443291705488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144570.19558246357061848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130150.048.2375831
GTATCAA219150.036.8480031
TACCTGG64850.030.6896462
GTACCTG67700.030.1683041
TCAACGC264700.029.3589274
GTACATG269650.029.2998941
ATCAACG268100.029.0047173
CAACGCA268700.028.9366195
AACGCAG279750.027.907046
TACATGG276750.027.8986022
TATCAAC280700.027.8244862
ACATGGG277300.026.5852363
ACGCAGA315250.024.5414457
CATGGGG172550.024.3706264
ACCTGGG82050.023.9367473
CGCAGAG324500.023.6548168
TATAACG7000.022.1808072
ATGGGGG98850.021.7673875
GCAGAGT354450.021.033149
GAGTACT216300.020.51473412-13
Produced by FastQC (version 0.11.5)