FastQCFastQC Report
Mon 6 Jun 2016
ERR1211589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7391767
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303780.4109707462369958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269700.36486539686654085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184120.24908793797207082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144800.047.8184051
GTATCAA244950.036.6764531
TACCTGG65750.032.1148832
GTACCTG67250.031.7502981
TCAACGC303450.028.7246024
CAACGCA306200.028.4660435
ATCAACG308200.028.3282093
AACGCAG316800.027.6187156
TATCAAC325100.027.1665422
GTACATG286100.027.0683381
TACATGG294950.025.9893232
ACCTGGG79900.025.818073
ACATGGG289600.025.571943
CATGGGG173400.025.228874
ACGCAGA362050.024.1017937
CGCAGAG370750.023.4475048
TAACGCA7750.023.0398224
ATGGGGG99400.022.5959135
TATAACG7600.022.2763822
GCAGAGT407800.021.0405279
Produced by FastQC (version 0.11.5)