FastQCFastQC Report
Mon 6 Jun 2016
ERR1211588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522256
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146510.3239754671119901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125000.27641071182171023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.18260797265789466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70450.046.2011641
GTATCAA123250.037.699371
TCAACGC147450.030.8815214
ATCAACG148300.030.7372263
CAACGCA149600.030.4032635
AACGCAG154800.029.5298126
TATCAAC158850.028.7572062
GTACATG143250.027.924541
TACATGG146550.026.3234442
ACGCAGA174650.025.9858517
CGCAGAG177250.025.2874458
ACATGGG147650.025.111793
TACCTGG36450.023.49032
CATGGGG83600.023.0460914
GTACCTG39050.022.4686371
GCAGAGT198600.021.740989
ATGGGGG50450.020.489815
AGAGTAC183400.019.6880110-11
GAGTACT130700.019.2688612-13
CAGAGTA196800.018.76526510-11
Produced by FastQC (version 0.11.5)