FastQCFastQC Report
Mon 6 Jun 2016
ERR1211588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4522256
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173420.38348116515296793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152360.33691148842524615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104820.23178696650521333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83900.045.9661981
GTATCAA141000.036.479841
TCAACGC171300.029.0497824
CAACGCA172350.028.8724885
ATCAACG172750.028.7790663
TATCAAC179800.027.9071372
AACGCAG179400.027.843256
GTACCTG36750.027.0690121
TACCTGG35800.026.6657372
GTACATG155100.025.3209761
TACATGG157800.024.347572
ACGCAGA205250.024.267617
ACATGGG155900.023.721263
CGCAGAG210100.023.662688
CATGGGG87850.023.0001664
GCAGAGT233950.021.0495369
GAGTACT150300.020.30622912-13
ACCTGGG46650.019.6422863
TAGGACG2902.7957867E-919.4440864
AGAGTAC217050.019.43027910-11
Produced by FastQC (version 0.11.5)