FastQCFastQC Report
Mon 6 Jun 2016
ERR1211587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128200.33322736945583864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108040.28082593600630895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70520.1833010459752398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.046.7152981
GTATCAA105500.036.7078971
TCAACGC124650.030.0088234
ATCAACG125850.029.6468493
CAACGCA127050.029.512475
AACGCAG131100.028.6679676
TATCAAC134150.028.0924632
GTACATG124750.028.0564121
TATAACG3850.026.884992
TACATGG126050.026.8370042
TAACGCA3750.026.3157774
ACATGGG127700.025.5375843
ACGCAGA147950.025.2451937
TACCTGG29750.024.6709352
CGCAGAG151900.024.5274968
GTACCTG31950.024.3602561
CATGGGG74900.023.653084
GTATAAC10850.021.7374731
GCAGAGT169450.021.5155439
ATAACGC4650.021.2215733
Produced by FastQC (version 0.11.5)