FastQCFastQC Report
Mon 6 Jun 2016
ERR1211587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146790.38154793730438813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128980.33525480586906453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90630.2355725155521268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72650.044.70221
GTATCAA118100.034.7627071
TACCTGG30600.028.585592
GTACCTG33100.027.9108721
TCAACGC148200.027.4594734
CAACGCA148900.027.3612295
ATCAACG149200.027.3394853
AACGCAG154450.026.5308446
TATCAAC156000.026.1969572
GTACATG131300.026.1343651
TACATGG135300.024.6435952
ACATGGG131400.024.4624613
CATGGGG74450.023.9850924
ACGCAGA174800.023.388397
CGCAGAG179150.022.8204888
TATAACG3200.022.044332
GCAGAGT197850.020.449569
ACCTGGG40950.020.4270463
GAGTACT128200.020.08660512-13
ATGGGGG43000.019.8890555
Produced by FastQC (version 0.11.5)