FastQCFastQC Report
Mon 6 Jun 2016
ERR1211586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9254693
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273460.2954825189771287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230290.24883591492446047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142230.153684190280542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139450.048.3479041
GTATCAA240800.038.7480131
TCAACGC282100.031.8178164
ATCAACG283300.031.7504253
CAACGCA283800.031.6065645
AACGCAG296900.030.270566
TATCAAC305150.029.9373052
GTACCTG79450.028.3657361
GTACATG296150.028.1946641
TACATGG300950.027.3696142
ACATGGG300750.026.7048783
TACCTGG80300.026.4788882
ACGCAGA336750.026.3957447
CGCAGAG342850.025.6937168
CATGGGG184350.024.2679944
GCAGAGT387100.021.9432189
ACCTGGG99850.020.9913793
ATGGGGG114000.020.5261845
GAGTACT248950.019.91263612-13
AGAGTAC356300.019.33882310-11
Produced by FastQC (version 0.11.5)