FastQCFastQC Report
Mon 6 Jun 2016
ERR1211586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9254693
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307560.3323286898873901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265750.2871516105396473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172300.1861758137195907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152450.046.7229961
GTATCAA256550.036.4371451
TCAACGC309850.029.6179854
ATCAACG311700.029.412523
CAACGCA315250.029.1555215
AACGCAG325050.028.3783176
TACCTGG79200.028.0252932
TATCAAC332900.027.8141172
GTACATG318850.027.6618371
GTACCTG83100.027.4560451
TACATGG325500.026.5105322
ACATGGG321800.025.6857093
ACGCAGA371150.024.6762317
CATGGGG195650.024.4016174
CGCAGAG380250.023.9868268
ATGGGGG113400.021.6718375
ACCTGGG101050.021.4841143
GCAGAGT427450.020.9094059
GAGTACT269600.019.66084512-13
AGAGTAC393150.019.34509710-11
Produced by FastQC (version 0.11.5)