FastQCFastQC Report
Mon 6 Jun 2016
ERR1211585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10103871
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258280.2556247996436217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222650.22036108734959106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142690.14122310152217896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147850.046.387841
GTATCAA252400.035.9938241
TCAACGC285950.030.464654
ATCAACG288550.030.1256073
CAACGCA288850.030.0905915
AACGCAG302400.028.8316526
GTACATG315600.028.2628291
TATCAAC314300.027.935122
TACATGG327100.026.4248092
ACATGGG322250.025.8232423
ACGCAGA344550.025.086687
CGCAGAG353200.024.2742398
GTACCTG83450.023.061891
CATGGGG183550.023.0390554
TACCTGG82650.022.259592
GCAGAGT397050.021.060869
GAGTACT249850.019.35994712-13
CAGAGTA385300.018.99783510-11
AGAGTAC363700.018.79556510-11
GAGTACA230400.017.9751761
Produced by FastQC (version 0.11.5)