FastQCFastQC Report
Mon 6 Jun 2016
ERR1211585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10103871
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303450.3003304377104577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266610.2638691646003794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178680.17684311290197588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160700.045.5980531
GTATCAA269700.037.0127261
TCAACGC323100.030.0885854
ATCAACG327800.029.6862813
CAACGCA329550.029.471175
AACGCAG342400.028.5581076
TATCAAC354200.027.7740782
GTACATG332450.026.154461
TACATGG341400.024.9862062
ACGCAGA391600.024.8736297
ACATGGG336450.024.4819533
CGCAGAG402650.024.0859898
GTACCTG77550.022.4365311
TACCTGG77300.021.7786062
CATGGGG191550.021.6844464
GCAGAGT453500.021.0537249
AGAGTAC418300.019.60715110-11
GAGTACT280250.019.55738812-13
AGTACTT299200.018.75057212-13
GTATAGG37000.018.6063981
Produced by FastQC (version 0.11.5)