FastQCFastQC Report
Mon 6 Jun 2016
ERR1211584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414468
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181600.28310999446875407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156160.24344965163128102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102740.16016916757554953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97800.044.960191
GTATCAA166200.037.130241
TACCTGG58950.032.483772
GTACCTG61400.031.8114171
TCAACGC195200.030.6671034
CAACGCA198350.030.2009435
ATCAACG198350.030.1561553
AACGCAG205800.029.1277666
TATCAAC207150.029.0496862
GTACATG198450.027.0784451
TACATGG201300.025.9438882
ACGCAGA232600.025.4891247
ACATGGG203900.024.8647423
CGCAGAG238700.024.7200458
ACCTGGG75850.024.6551273
ATAACGC5650.024.1173323
CATGGGG129350.022.8126834
GCAGAGT265500.021.4460229
TATAACG5950.020.5594842
GAGTACT164800.020.05623212-13
Produced by FastQC (version 0.11.5)