FastQCFastQC Report
Mon 6 Jun 2016
ERR1211584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6414468
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215120.3353668612891981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194080.3025660117097786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131880.2055977206527494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.044.8848651
GTATCAA178300.035.1662251
GTACCTG62450.032.6119461
TACCTGG62150.031.5565152
TCAACGC218500.028.0651344
CAACGCA220850.027.7662855
ATCAACG222100.027.6959363
AACGCAG231550.026.5850776
TATCAAC232800.026.5464552
TATAACG7350.025.5956862
GTACATG216000.025.0342791
ACCTGGG77350.024.7870483
TACATGG218900.024.0424332
ACATGGG219950.023.0527343
ATAACGC7750.023.0412923
ACGCAGA266150.023.0230337
CGCAGAG273650.022.3233478
TAACGCA7900.022.0081044
CATGGGG134400.021.5372124
GCAGAGT307950.019.5773779
Produced by FastQC (version 0.11.5)